Comparison of probiotic properties of bacteria isolated from intestinal Rutilus kutum of Caspian Sea with imported probiotics

Abstract

Introduction: Lactic acid bacteria play an important role in animal health. The aim of this study was to investigate the probiotic properties of bacteria isolated from Rutilus kutum intestine and compare it with imported probiotics. Materials & Methods: In this study, Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus brevis were isolated from Rutilus kutum intestines by phenotypic and PCR methods and identified with imported probiotics (Lactobacillus bulgaris prepared by Kristin Hansen, Denmark), compared to Denmark. They were paid. Results: First, fermentation of sugars, in which Lactobacillus plantarum has the highest number of sugars, including sugars; Fermented arabinose, sucrose, raffinose, cellobiose, ribose, glucose, fructose, galactose, trehalose, lactose, mannose, mannitol, melobiose and melocytose Fermented glucose, fructose, trehalose, lactose and melocytosis. Survival of probiotics under acidic conditions has been as follows; All Lactobacillus except Lactobacillus plantarum had a resistance percentage (43%) at pH=3-4. However, other isolated lactobacilli and imported probiotics had a good resistance percentage (65% -75%) of growth under acidic conditions. Compared with bile salt test, Lactobacillus brevis did not grow in 0.6% of bile salts, but other imported Lactobacillus and probiotics did not grow well in different concentrations (0.4%, 0.5% and 0.6%). They had bile salts. Compared to the susceptibility test, imported probiotics were sensitive to all antibiotics, but isolated lactobacilli were resistant to the vancomycin antibiotic. The inhibitory effect of isolated and imported lactic acid bacteria on Escherichia coli and Pseudomonas aeruginosa was also investigated by disk diffusion method. In order to reduce the error, each test was repeated at least three times. The maximum mean diameter of growth inhibition zone for Lactobacillus acidophilus on Escherichia coli and Sodomonas aeruginosa was 12 and 14 mm, respectively. Has not created growth. The diameter of the growth probe of imported probiotics on Escherichia coli and Pseudomonas aeruginosa was 9 and 5 mm, respectively. In the next comparison, lactobacilli isolated from the intestines of Rutilus kutum and imported probiotics were added to the diet of salmon infected with the bacterium Aeromonas hydrophila (injection into the peritoneum of fish), which caused a significant reduction in 21 days. We have witnessed the death of salmon. Conclusion: Lactobacillus isolated from whitefish intestines have more probiotic potential than imported bacteria (Lactobacillus bulgaris) and is a very suitable alternative for use in aquaculture and industry.