Effect of Low-Level Pulsed Laser-Assisted Processing of DNA-Modified Nano Raw Cellulose Obtained from Phoenix dactylifera Rachis on Achilles Tendon Healing in Rabbits.

Abstract

Injuries of the Achilles tendon have a protracted healing process due to poor blood supply, low cell count, disorganised collagen orientation and reduced mechanical strength.This study examines the impact of low level pulsed laser assistance Phoenix dactylifera rachis raw nanocellulose on Achilles tendon healing in rabbits was evaluated in the presence of bacterial genomic DNA. Thirty senior New Zealand White rabbits were subjected to total Achilles tendon transection followed by modified Kessler repair and were split up into two equal groups at random.The control group wasn’t treated just suturing  whereas the experimental group was treated by 0.3 ML of DNA-modified raw nanocellulose Suspension , applied topically at the site of repair. The nanocellulose was characterised by FESEM, FTIR, XRD and UV-Vis spectroscopy. Healing was examined clinically, ultrasonographically, histopathologically and biomechanically at 0, 7, 14, 30 and 60 days postoperatively. FESEM confirmed the presence of nanoscale particles ranging from 25.81 to 41.28 nm. Compared to the control group, the treated group displayed greater clinical improvement, reduced inflammatory parameters, higher ultrasonographic tendon continuity, more structured collagen deposition, and improved mechanical strength. The results indicated that nanocellulose from the raw date palm rachis, obtained by low-level pulsed laser-assisted processing, could be a promising local biomaterial for experimental tendon rehabilitation.