Isolation and Molecular Identification of Uropathogenic Organisms from Patients with UTIs

Abstract

Urinary infections, commonly caused by bacterial pathogens, affect individuals across all age groups, with women, infants, and the elderly being more susceptible. This study isolated and identified bacterial strains from urine samples of 50 UTI patients under sterile conditions using different culturing media. A total of 59 distinct bacterial strains underwent gram staining and biochemical tests like Indole, Methyl Red, Voges-Proskauer, Citrate Utilization, Urease, Catalase, Oxidase, Ammonia Production, Motility and TSI. Biochemical test results revealed that 49.15% were Gram-positive and the rest were Gram-negative strains, 89.83% were Indole-positive, 79.66% were Methyl Red-positive, 61.02% were Voges-Proskauer-positive, 72.88% were positive for citrate utilization, 25.42% of strains were urease positive, 0% Catalase-positive and motile, 18.64% were Oxidase-positive, 47.46% produced Ammonia, and 30.51% produced gas in TSI tests. These biochemical tests revealed the isolated organisms to be Klebsiella oxytoca, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi, Vibrio cholera, Citrobacter koseri. The remaining cultures that cannot be identified were identified using molecular techniques the species were found to be Clostridium sp, Pseudomoans sps, Bacillus megaterium, Desemzia incerta, Exiguobacterium sp., Cellulosimicrobium funkei, Paraclostridium benzoelyticum, Proteus mirabilis, Lysinibacillus fusiformis, Lysinibacillus macroides, Pseudomonas sp., and Uncultured bacterium. Based on the above observation various pathogens are involved in causing urinary tract infections revealing its complexity and the need for effective management.