Evaluation of cervical cytology in estrous postpartum anestrous cows has been treated according to CoSynch protocol with normal dairy cows

Abstract

Introduction: Cytological examination of the reproductive tract is often used to
evaluate possible reproductive lesions in humans and domestic animals. Cytological
method has been used to determination of the subclinical endometritis in the cattle.
There was no information about cytological cervical cervix in anestrous cow. The aim
of this study was to compare cervical cytology between normal postpartum and anestrus
cow that treat by ovulation synchronization in heat day.
Materials & Methods: This prospective cohort study was performed on 50 estrous
Holstein cows with various days in milk (DIM) and parity (2-4), in a moderate
commercial dairy herd in susangerd, of Ahvaz province, southern Iran, from April to
December 2022. Cervical mucus was aspirated with a pipette. Giemsa stain Cytological
assessment determined the percent neutrophils (percentage PMN) by counting a
minimum of 100 cells at 400× magnification, in order to provide a quantitative
assessment of ratio %PMN to % epithelial cell.
Results: No significant differences were observed in ratio % PMN with %epithelial cell
in smears of cows between anestrus cow that treatment by CoSynch and control in heat
day. As well as, in our study the mean neutrophil percentage according to the parity of
cows between the two groups done. Although the neutrophil percentage was higher in
parity 2 in the CoSynch group, this difference was not significant (p≥ 0.05).
Conclusion: The findings indicate that anestrus dairy cow treated with synchronization
of ovulation protocols in estrus day no difference of % PMN with %epithelial cell ratio
in cytological smears.